谭树华

谭树华中国药科大学生化药学博士,美国University of Nebraska Medical Center医学细胞与分子生物学博士后,教授,博士生导师。历任中国药科大学生物制药教研室副教授、副主任、主任,江苏省生化与分子生物学会理事。现任分子生物学教研室主任,江苏省生物技术协会常务理事、副秘书长国家自然科学基金项目审评专家,国家教育部留学回国人员科研基金评审专家, 多个国际SCI期刊杂志编委及审稿人。

研究领域为生物制药,研究方向是生物药学基础与应用研究,主要开展重组多肽、蛋白及抗体等生物大分子创新药物的基因工程与蛋白质工程,及其关键技术研究,同时在分子、细胞及动物水平研究药物作用机制并发现新作用靶点。先后主持承担了国家新药研究基金项目、国家自然科学基金青年基金项目、国家自然科学基金面上项目、国家重大新药创制科技重大专项十一五十二五计划项目、国家教育部霍英东教师奖励基金项目、国家教育部留学回国人员基金项目、江苏省 “六大人才高峰项目、江苏省科技厅项目、 南京市留学人员科技活动择优资助项目、 技术成果转化及校企合作开发项目等。

在国际SCI及国内核心学术期刊发表学术论文50余篇, 获得国家及省级鉴定成果3项(主持1项,参加2项),试生产批文1项,申请中国发明专利12项。荣获第七届江苏省青年科技奖、江苏省科技进步三等奖、江苏省轻工业厅科技进步特等奖、美国University of Nebraska Medical Center杰出表现津贴奖(FY2005~06)等奖项。入选江苏省“333”新世纪学术带头人。

讲授本科生分子生物学生物药学基础与前沿(新生研讨课)、中英合作班“Cell and Molecular Biology”等课程,以及研究生药物生物技术研究进展、留学硕士生“Advances in Molecular Biology”等课程。为本科生讲授的生物工程课程荣获江苏省高等学校I类优秀课程奖及精品课程奖,指导研究生完成的科技作品荣获全国第七届大学生挑战杯竞赛一等奖。

主编《药学分子生物学》(中国医药科技出版社)、《分子生物学实验指导》(中国医药科技出版社),副主编《生物技术制药概论》(中国医药科技出版社)、《生物制药技术》(化学工业出版社),共同编写《生物工程》(中国医药科技出版社)。


研究方向

1.生物新药的基础与应用研究

2.基因工程与蛋白质工程关键技术研究

3.药物相关基因的表达与调控研究

4.生物药物靶向分子与技术研究


联系方式

1.E-mail: tohike@hotmail.com ; tanshuhua126@126.com

2.电话:025-86185253

3.手机:15850538060

4.通讯地址:南京市江宁区龙眠大道639号,中国药科大学(邮编:211198


主要论文

1.L. Gu, P. Ye, H. Li, Y. Wang, Y. Xu, Q. Tian, G. Lei, C. Zhao, Z. Gao, W. Zhao, S. Tan*, Lunasin attenuates oxidant-induced endothelial injury and inhibits atherosclerotic plaque progression in ApoE(-/-) mice by up-regulating heme oxygenase-1 via PI3K/Akt/Nrf2/ARE pathway. FASEB J 33 (2019) 4836-4850.

2.G. Lei, M. Xu, Z. Xu, C. Lu, S. Tan*, Combination of novel DR5 targeting agonistic scFv antibody TR2-3 with cisplatin shows enhanced synergistic antitumor activity in vitro and in vivo. Biomed Pharmacother 98 (2018) 271-279.

3.L. Gu, Y. Wang, Y. Xu, Q. Tian, G. Lei, C. Zhao, Z. Gao, Q. Pan, W. Zhao, L. Nong, S Tan*, Lunasin functionally enhances LDL uptake via inhibiting PCSK9 and enhancing LDLR expression in vitro and in vivo,Oncotarget 8 (2017) 80826-80840.

4.G. Lei, M. Xu, Z. Xu, L. Gu, C. Lu, Z. Bai, Y. Wang, Y. Zhang, H. Hu, Y. Jiang, W. Zhao, S. Tan*, A Novel Fully Human Agonistic Single Chain Fragment Variable Antibody Targeting Death Receptor 5 with Potent Antitumor Activity In Vitro and In Vivo, Int J Mol Sci, 18 (2017).

5.Q. Tian, P. Zhang, Z. Gao, H. Li, Z. Bai, S. Tan*, Hirudin as a novel fusion tag for efficient production of lunasin in Escherichia coli, Prep Biochem Biotechnol, 47 (2017) 619-626.

6.J. Zhu, Q. Zhou, S. Tan*, Targeting miRNAs associated with surface expression of death receptors to modulate TRAIL resistance in breast cancer, Cancer Lett, 383 (2016) 154-160.

7.G. Dai, P. Zhang, P. Ye, M. Zhang, N. Han, H. Shuai, S. Tan*, The Chemopreventive Peptide Lunasin Inhibits d-Galactose- Induced Experimental Cataract in Rats, Protein Pept Lett, 23 (2016) 619-625.

8.S. Setrerrahmane, Y. Zhang, G. Dai, J. Lv, S. Tan*, Efficient production of native lunasin with correct N-terminal processing by using the pH-induced self-cleavable Ssp DnaB mini-intein system in Escherichia coli, Appl Biochem Biotechnol, 174 (2014) 612-622.

9.X. Gong, Q. Zhang, S. Tan*, Inhibitory effect of r-hirudin variant III on streptozotocin-induced diabetic cataracts in rats, ScientificWorldJournal, 2013 (2013) 630651.

10.H. Zhang, P. Dramou, H. He, S. Tan*, C. Pham-Huy, H. Pan, Molecularly imprinted stationary phase prepared by reverse micro-emulsion polymerization for selective recognition of gatifloxacin in aqueous media, J Chromatogr Sci, 50 (2012) 499-508.

11.L. Shen, Z. Liu, F. Xie, X. Zhang, S. Tan*, A novel approach for secretion of heterologous proteins with correct n-terminal processing by using alpha-factor pre sequence in Pichia pastoris, Protein Pept Lett, 19 (2012) 1005-1009.

12.J. Lv, C. Huang, X. Zhang, S. Tan*, Extracellular secretion of anticoagulant peptide hirudin in Lactococcus lactis using SP310mut2 signal peptide, Biotechnol Lett, 34 (2012) 61-65.

13.C. Huang, X. Zhang, J. Qu, P. Zhang, S. Tan*, Robust preparative-scale extracellular production of hirudin in Escherichia coli and its purification and characterization, J Ind Microbiol Biotechnol, 39 (2012) 1487-1494.

14.Z. Chen, D. Pierre, H. He, S. Tan*, C. Pham-Huy, H. Hong, J. Huang, Adsorption behavior of epirubicin hydrochloride on carboxylated carbon nanotubes, Int J Pharm, 405 (2011) 153-161.

15.S. Tan*, W. Wu, X. Li, L. Cui, B. Li, Q. Ruan, Enhanced secretion of adhesive recognition sequence containing hirudin III mutein in E. coli, Mol Biotechnol, 36 (2007) 1-8.

16.S. Tan, P.W. Cheng, Mucin biosynthesis: identification of the cis-regulatory elements of human C2GnT-M gene, Am J Respir Cell Mol Biol, 36 (2007) 737-745.

17.P. Radhakrishnan, P.V. Beum, S. Tan, P.W. Cheng, Butyrate induces sLex synthesis by stimulation of selective glycosyltransferase genes, Biochem Biophys Res Commun, 359 (2007) 457-462.

18.M. Hashimoto, S. Tan, N. Mori, H. Cheng, P.W. Cheng, Mucin biosynthesis: molecular cloning and expression of mouse mucus-type core 2 beta1,6 N-acetylglucosaminyltransferase, Glycobiology, 17 (2007) 994-1006.

19.K.N. Nemec, A.H. Pande, S. Qin, R.J. Bieber Urbauer, S. Tan, D. Moe, S.A. Tatulian, Structural and functional effects of tryptophans inserted into the membrane-binding and substrate-binding sites of human group IIA phospholipase A2, Biochemistry, 45 (2006) 12448-12460.

20.A.H. Pande, D. Moe, K.N. Nemec, S. Qin, S. Tan, S.A. Tatulian, Modulation of human 5-lipoxygenase activity by membrane lipids, Biochemistry, 43 (2004) 14653-14666.

21.S. Tan, W. Wu, J. Liu, Y. Kong, Y. Pu, R. Yuan, Efficient expression and secretion of recombinant hirudin III in E. coli using the L-asparaginase II signal sequence, Protein Expr Purif, 25 (2002) 430-436.